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mouse neuroblastoma cell line n2a  (ATCC)


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    ATCC mouse neuroblastoma cell line n2a
    Mouse Neuroblastoma Cell Line N2a, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 4218 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+neuro+2a+n2a+neuroblastoma+cell+line/Neuro-2a/pm42120407-428-0-5
    Average 99 stars, based on 4218 article reviews
    mouse neuroblastoma cell line n2a - by Bioz Stars, 2026-09
    99/100 stars

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    Modification:

    Article Title: Prevention by Dietary Polyphenols (Resveratrol, Quercetin, Apigenin) Against 7-Ketocholesterol-Induced Oxiapoptophagy in Neuronal N2a Cells: Potential Interest for the Treatment of Neurodegenerative and Age-Related Diseases
    Article Snippet: .. The mouse neuro-2a (N2a) neuroblastoma cell line (Ref: CCL-131, American Type Culture Collection (ATCC), Manassas, VA, USA) was maintained in Dulbecco’s modified Eagle medium (DMEM, Lonza, Amboise, France) containing 10% (v/v) of heat-inactivated fetal bovine serum (FBS) (Pan Biotech, Aidenbach, Germany) (30 min, 56 °C) and 1% (v/v) of penicillin (100 U/mL)/streptomycin (100 mg/mL) (Pan Biotech). ..

    Article Title: Cytotoxic and Antioxidant Activities of Imine Analogs of Trans-Resveratrol towards Murine Neuronal N2a Cells
    Article Snippet: .. The mouse neuro-2a (N2a) neuroblastoma cell line (Ref: CCL-131, American Type Culture Collection (ATCC), Manassas, VA, USA) was maintained in Dulbecco’s modified Eagle medium (DMEM, Lonza, Amboise, France) containing 10% ( v/v ) of heat-inactivated fetal bovine serum (FBS) (Pan Biotech, Aidenbach, Germany) (30 min, 56 °C) and 1% ( v/v ) of penicillin (100 U/mL)/streptomycin (100 mg/mL) (Pan Biotech). ..

    Cell Culture:

    Article Title: Characterization of Cell Death Induced by Imine Analogs of Trans-Resveratrol: Induction of Mitochondrial Dysfunction and Overproduction of Reactive Oxygen Species Leading to, or Not, Apoptosis without the Increase in the S-Phase of the Cell Cycle
    Article Snippet: .. The mouse neuro-2a (N2a) neuroblastoma cell line (Ref: CCL-131, American Type Culture Collection (ATCC), Manassas, VA, USA) was cultured as previously described [ ]. .. The cells were cultured in Dulbecco’s modified Eagle medium (DMEM, Lonza, Amboise, France), which contains 10% ( v/v ) of heat-inactivated fetal bovine serum (FBS) (Pan Biotech, Aidenbach, Germany) and 1% ( v/v ) of penicillin (100 U/mL)/streptomycin (100 mg/mL) (Pan Biotech).

    Article Title: Phospho-substrate profiling of Epac-dependent protein kinase C activity
    Article Snippet: .. Cell culture and reagents The mouse Neuro-2a (N2A) neuroblastoma cell line was purchased from ATCC (ATCC Number: CCL-131). .. Cells were propagated and cultured at 37° C/5% CO2 in complete media (Minimal Essential Media, GlutaMAXTM (MEM: Gibco-ThermoFisher, Waltham, MA) supplemented with 10% heat inactivated Fetal Bovine Serum (Gibco-ThermoFisher, Waltham, MA) and 1% Penicillin-Streptomycin Solution (Corning, Tewksbury, MA)).

    Article Title: Characterization of Cell Death Induced by Imine Analogs of Trans-Resveratrol: Induction of Mitochondrial Dysfunction and Overproduction of Reactive Oxygen Species Leading to, or Not, Apoptosis without the Increase in the S-Phase of the Cell Cycle.
    Article Snippet: .. The mouse neuro-2a (N2a) neuroblastoma cell line (Ref: CCL-131, American Type Culture Collection (ATCC), Manassas, VA, USA) was cultured as previously described [15]. .. The cells were cultured in Dulbecco’s modified Eagle medium (DMEM, Lonza, Amboise, France), which contains 10% (v/v) of heat-inactivated fetal bovine serum (FBS) (Pan Biotech, Aidenbach, Germany) and 1% (v/v) of penicillin (100 U/mL)/streptomycin (100 mg/mL) (Pan Biotech).

    other:

    Article Title: Neuroprotective Effect of Riboflavin Producing Lactic Acid Bacteria in Parkinsonian Models.
    Article Snippet: Oxidative stress and inflammatory processes might contribute to the cascade of events leading Parkinson disease (PD); and vitamins such as riboflavin can exert protection on vulnerable neurons in neurodegenerative conditions.. Previously, it was demonstrated that a mixture of lactic acid bacteria (including a riboflavin-producing strain) improved motor skills in a parkinsonian model.. The aim of the present work was to investigate the neuroprotective potential of Lactiplantibacillus (L.) plantarum CRL2130, a riboflavin-producing strain in PD models.



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    Abcam ab64186 recognizes NRN1 protein in mouse <t>neuroblastoma</t> cells. (A) Representative Western blot of human embryonic kidney (HEK) <t>293T,</t> <t>Neuro‐2a</t> <t>(N2a)</t> mouse neuroblastoma, and SH‐SY5Y human neuroblastoma cell lysates (50 µg protein), probed with NRN1 polyclonal antibody Abcam ab64186 and GAPDH monoclonal antibody. (B) N2a cells were transfected with NRN1 or Scramble (non‐targeting) siRNA smart pools and harvested after 96 h. Western blot analyses with NRN1 polyclonal antibody Abcam ab64186 revealed reduced intensity of the ∼34 kDa band in NRN1‐depleted cells, compared to Scramble control. 10 µg of protein was loaded per lane, and GAPDH was probed as a loading control. GAPDH, glyceraldehyde‐3‐phosphate dehydrogenase; NRN1, Neuritin‐1; siRNA, small interfering RNA;
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    Abcam ab64186 recognizes NRN1 protein in mouse neuroblastoma cells. (A) Representative Western blot of human embryonic kidney (HEK) 293T, Neuro‐2a (N2a) mouse neuroblastoma, and SH‐SY5Y human neuroblastoma cell lysates (50 µg protein), probed with NRN1 polyclonal antibody Abcam ab64186 and GAPDH monoclonal antibody. (B) N2a cells were transfected with NRN1 or Scramble (non‐targeting) siRNA smart pools and harvested after 96 h. Western blot analyses with NRN1 polyclonal antibody Abcam ab64186 revealed reduced intensity of the ∼34 kDa band in NRN1‐depleted cells, compared to Scramble control. 10 µg of protein was loaded per lane, and GAPDH was probed as a loading control. GAPDH, glyceraldehyde‐3‐phosphate dehydrogenase; NRN1, Neuritin‐1; siRNA, small interfering RNA;

    Journal: Alzheimer's & Dementia

    Article Title: NRN1 as a therapeutic target for Alzheimer's disease

    doi: 10.1002/alz.71149

    Figure Lengend Snippet: Abcam ab64186 recognizes NRN1 protein in mouse neuroblastoma cells. (A) Representative Western blot of human embryonic kidney (HEK) 293T, Neuro‐2a (N2a) mouse neuroblastoma, and SH‐SY5Y human neuroblastoma cell lysates (50 µg protein), probed with NRN1 polyclonal antibody Abcam ab64186 and GAPDH monoclonal antibody. (B) N2a cells were transfected with NRN1 or Scramble (non‐targeting) siRNA smart pools and harvested after 96 h. Western blot analyses with NRN1 polyclonal antibody Abcam ab64186 revealed reduced intensity of the ∼34 kDa band in NRN1‐depleted cells, compared to Scramble control. 10 µg of protein was loaded per lane, and GAPDH was probed as a loading control. GAPDH, glyceraldehyde‐3‐phosphate dehydrogenase; NRN1, Neuritin‐1; siRNA, small interfering RNA;

    Article Snippet: Neuro‐2a (N2a) mouse neuroblastoma cell lines (Catalog No.: CCL‐131, ATCC) were maintained in MEM (Catalog No.: 11095‐080, Thermo Fisher Scientific) with 10% FBS and 1% penicillin‐streptomycin.

    Techniques: Western Blot, Transfection, Control, Small Interfering RNA